anti crel  (Santa Cruz Biotechnology)

Journal: RSC Medicinal Chemistry

Article Title: Design, synthesis and evaluation of pyrrolobenzodiazepine (PBD)-based PROTAC conjugates for the selective degradation of the NF-κB RelA/p65 subunit †

doi: 10.1039/d5md00316d

Figure Lengend Snippet: Comparison the effect of 15d (PROTAC) on the expression of three NF-κB subunits RelA/p65, RelB and cRel. Each subunit was quantified using fluorescence-labelled antibodies; all experiments were performed three times in duplicate and data are presented as violin plots. Statistical significance was determined using the Kruskal–Wallis test with Dunn's multiple comparison post hoc correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Cells were then washed in Cyto-FastTM Perm Wash solution and centrifuged at 300 × g for 5 min before being resuspended in 100 μL Perm Wash solution followed by the addition of 5 μL APC-labelled RelA/p65 antibody (Biolegend), 5 μL PE-labelled cRel antibody (eBiosciences) and 5 μL corallite 488-labelled RelB antibody (ThermoFisher).

Techniques: Comparison, Expressing, Fluorescence

Journal: Cell

Article Title: Thyroid hormone remodels cortex to coordinate body-wide metabolism and exploration

doi: 10.1016/j.cell.2024.07.041

Figure Lengend Snippet:

Article Snippet: DRH032_ AAV-SIO-nEF-DN-THR , This paper , Addgene 225091.

Techniques: Virus, Plasmid Preparation, Recombinant, Control, Protease Inhibitor, RNAscope, Multiplex Assay, Negative Control, Software, Modification

Journal: Cell

Article Title: Thyroid hormone remodels cortex to coordinate body-wide metabolism and exploration

doi: 10.1016/j.cell.2024.07.041

Figure Lengend Snippet:

Article Snippet: DRH031_ AAV-SIO-nEF-WT-THR , This paper , Addgene 225090.

Techniques: Virus, Plasmid Preparation, Recombinant, Control, Protease Inhibitor, RNAscope, Multiplex Assay, Negative Control, Software, Modification